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How to obtain a blood sample in reptiles via venepuncture

02 November 2014
8 mins read
Volume 5 · Issue 9

Abstract

The use of venepuncture in reptiles is an important diagnostic step in determining illness and disease. There are several venepuncture sites that vary between species and knowledge of these will ensure a diagnostic sample. Knowing how to correctly handle the reptile will prevent any unnecessary injury to the handler or the animal. The correct gauge needle and smallest syringe need to be used so as not to damage the red blood cells, and the site needs to be cleaned appropriately. This article will look at the venepuncture sites and techniques used in the Chelonian and Squamata species.

There are approximately 9000 known species of reptile (Rendle and Crack-nell, 2012). These are subdivided into four categories:

  • Chelonia: turtles, tortoises and terrapins
  • Squamata:
  • ◦ Sauria — lizards
  • ◦ Serpentes — snakes
  • Crocodilia: alligators and crocodiles
  • Rhynchocephalia: tuatara.
  • Reptiles can be difficult to clinically assess and a blood sample can be an important diagnostic step. A blood profile may include:

  • Renal parameters: high levels of uric acid may indicate renal disease
  • Glucose levels: may indicate hypoglycaemia in a malnourished animal. Hyperglycaemia in bearded dragons can be indicative of neoplasia
  • Calcium and phosphorous levels: can indicate if a female is gravid or if the animal is suffering from nutritional secondary hyperparathyroidism (NSHP)
  • Haematology: high or low white-cell count can indicate a bacterial or viral disease
  • Hydration levels: high urea may indicate dehydration (e.g. in tortoises)
  • Total protein levels: indicate liver function and nutritional stasis.
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